cat sk Search Results


sk-mel-28 (cat. no gcc-me0003cs)  (Shanghai Genechem Ltd)
90
Shanghai Genechem Ltd sk-mel-28 (cat. no gcc-me0003cs)
Effects of Linc00961 on the proliferation and apoptosis of A375 and <t>SK-MEL-28</t> cells. Effects of Linc00961 on the proliferation of (A) A375 and (B) SK-MEL-28 cells, as determined using a Cell Counting Kit-8 assay. (C) Effects of Linc00961 on the apoptosis of A375 and SK-MEL-28 cells, detected via flow cytometry. (D) Statistical analysis of the early apoptotic rates of A375 and SK-MEL-28 cells infected with Lv-control and Lv-Linc00961. (E) Tumor growth curve of A375 cells transfected with Lv-control and Lv-Linc00961. (F) Digital pictures of the resected tumors of animals injected with A375 cells infected with Lv-control (n=3) and Lv-Linc00961 (n=3), captured after 35 days. The maximum diameters of Lv-control and Lv-Linc00961 were 1.718 and 0.737 cm, respectively. Scale bar=1 cm. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. Lv-control. Linc00961, long intergenic noncoding RNA 00961; Lv, lentivirus; PI, propidium iodide.
Sk Mel 28 (Cat. No Gcc Me0003cs), supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sk-mel-28 (cat. no gcc-me0003cs)/product/Shanghai Genechem Ltd
Average 90 stars, based on 1 article reviews
sk-mel-28 (cat. no gcc-me0003cs) - by Bioz Stars, 2026-03
90/100 stars
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il17 neutralising antibodies secukinumab  (Creative Biolabs)
90
Creative Biolabs il17 neutralising antibodies secukinumab
Effects of Linc00961 on the proliferation and apoptosis of A375 and <t>SK-MEL-28</t> cells. Effects of Linc00961 on the proliferation of (A) A375 and (B) SK-MEL-28 cells, as determined using a Cell Counting Kit-8 assay. (C) Effects of Linc00961 on the apoptosis of A375 and SK-MEL-28 cells, detected via flow cytometry. (D) Statistical analysis of the early apoptotic rates of A375 and SK-MEL-28 cells infected with Lv-control and Lv-Linc00961. (E) Tumor growth curve of A375 cells transfected with Lv-control and Lv-Linc00961. (F) Digital pictures of the resected tumors of animals injected with A375 cells infected with Lv-control (n=3) and Lv-Linc00961 (n=3), captured after 35 days. The maximum diameters of Lv-control and Lv-Linc00961 were 1.718 and 0.737 cm, respectively. Scale bar=1 cm. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. Lv-control. Linc00961, long intergenic noncoding RNA 00961; Lv, lentivirus; PI, propidium iodide.
Il17 Neutralising Antibodies Secukinumab, supplied by Creative Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/il17 neutralising antibodies secukinumab/product/Creative Biolabs
Average 90 stars, based on 1 article reviews
il17 neutralising antibodies secukinumab - by Bioz Stars, 2026-03
90/100 stars
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ivm culture medium kitazato cat.# sk-ivmc-50  (Kitazato Corporation)
90
Kitazato Corporation ivm culture medium kitazato cat.# sk-ivmc-50
Effects of Linc00961 on the proliferation and apoptosis of A375 and <t>SK-MEL-28</t> cells. Effects of Linc00961 on the proliferation of (A) A375 and (B) SK-MEL-28 cells, as determined using a Cell Counting Kit-8 assay. (C) Effects of Linc00961 on the apoptosis of A375 and SK-MEL-28 cells, detected via flow cytometry. (D) Statistical analysis of the early apoptotic rates of A375 and SK-MEL-28 cells infected with Lv-control and Lv-Linc00961. (E) Tumor growth curve of A375 cells transfected with Lv-control and Lv-Linc00961. (F) Digital pictures of the resected tumors of animals injected with A375 cells infected with Lv-control (n=3) and Lv-Linc00961 (n=3), captured after 35 days. The maximum diameters of Lv-control and Lv-Linc00961 were 1.718 and 0.737 cm, respectively. Scale bar=1 cm. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. Lv-control. Linc00961, long intergenic noncoding RNA 00961; Lv, lentivirus; PI, propidium iodide.
Ivm Culture Medium Kitazato Cat.# Sk Ivmc 50, supplied by Kitazato Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ivm culture medium kitazato cat.# sk-ivmc-50/product/Kitazato Corporation
Average 90 stars, based on 1 article reviews
ivm culture medium kitazato cat.# sk-ivmc-50 - by Bioz Stars, 2026-03
90/100 stars
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conjugation reaction buffer kit sk-20  (Boston Biochem)
90
Boston Biochem conjugation reaction buffer kit sk-20
Effects of Linc00961 on the proliferation and apoptosis of A375 and <t>SK-MEL-28</t> cells. Effects of Linc00961 on the proliferation of (A) A375 and (B) SK-MEL-28 cells, as determined using a Cell Counting Kit-8 assay. (C) Effects of Linc00961 on the apoptosis of A375 and SK-MEL-28 cells, detected via flow cytometry. (D) Statistical analysis of the early apoptotic rates of A375 and SK-MEL-28 cells infected with Lv-control and Lv-Linc00961. (E) Tumor growth curve of A375 cells transfected with Lv-control and Lv-Linc00961. (F) Digital pictures of the resected tumors of animals injected with A375 cells infected with Lv-control (n=3) and Lv-Linc00961 (n=3), captured after 35 days. The maximum diameters of Lv-control and Lv-Linc00961 were 1.718 and 0.737 cm, respectively. Scale bar=1 cm. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. Lv-control. Linc00961, long intergenic noncoding RNA 00961; Lv, lentivirus; PI, propidium iodide.
Conjugation Reaction Buffer Kit Sk 20, supplied by Boston Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/conjugation reaction buffer kit sk-20/product/Boston Biochem
Average 90 stars, based on 1 article reviews
conjugation reaction buffer kit sk-20 - by Bioz Stars, 2026-03
90/100 stars
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monovalent, inactivated, unadjuvanted m. canis vaccines for use in the cat  (BIOVETA SK)
90
BIOVETA SK monovalent, inactivated, unadjuvanted m. canis vaccines for use in the cat
Effects of Linc00961 on the proliferation and apoptosis of A375 and <t>SK-MEL-28</t> cells. Effects of Linc00961 on the proliferation of (A) A375 and (B) SK-MEL-28 cells, as determined using a Cell Counting Kit-8 assay. (C) Effects of Linc00961 on the apoptosis of A375 and SK-MEL-28 cells, detected via flow cytometry. (D) Statistical analysis of the early apoptotic rates of A375 and SK-MEL-28 cells infected with Lv-control and Lv-Linc00961. (E) Tumor growth curve of A375 cells transfected with Lv-control and Lv-Linc00961. (F) Digital pictures of the resected tumors of animals injected with A375 cells infected with Lv-control (n=3) and Lv-Linc00961 (n=3), captured after 35 days. The maximum diameters of Lv-control and Lv-Linc00961 were 1.718 and 0.737 cm, respectively. Scale bar=1 cm. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. Lv-control. Linc00961, long intergenic noncoding RNA 00961; Lv, lentivirus; PI, propidium iodide.
Monovalent, Inactivated, Unadjuvanted M. Canis Vaccines For Use In The Cat, supplied by BIOVETA SK, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monovalent, inactivated, unadjuvanted m. canis vaccines for use in the cat/product/BIOVETA SK
Average 90 stars, based on 1 article reviews
monovalent, inactivated, unadjuvanted m. canis vaccines for use in the cat - by Bioz Stars, 2026-03
90/100 stars
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4–0 silk cat #683-sk  (Henry Schein Inc)
90
Henry Schein Inc 4–0 silk cat #683-sk
Effects of Linc00961 on the proliferation and apoptosis of A375 and <t>SK-MEL-28</t> cells. Effects of Linc00961 on the proliferation of (A) A375 and (B) SK-MEL-28 cells, as determined using a Cell Counting Kit-8 assay. (C) Effects of Linc00961 on the apoptosis of A375 and SK-MEL-28 cells, detected via flow cytometry. (D) Statistical analysis of the early apoptotic rates of A375 and SK-MEL-28 cells infected with Lv-control and Lv-Linc00961. (E) Tumor growth curve of A375 cells transfected with Lv-control and Lv-Linc00961. (F) Digital pictures of the resected tumors of animals injected with A375 cells infected with Lv-control (n=3) and Lv-Linc00961 (n=3), captured after 35 days. The maximum diameters of Lv-control and Lv-Linc00961 were 1.718 and 0.737 cm, respectively. Scale bar=1 cm. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. Lv-control. Linc00961, long intergenic noncoding RNA 00961; Lv, lentivirus; PI, propidium iodide.
4–0 Silk Cat #683 Sk, supplied by Henry Schein Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/4–0 silk cat #683-sk/product/Henry Schein Inc
Average 90 stars, based on 1 article reviews
4–0 silk cat #683-sk - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Effects of Linc00961 on the proliferation and apoptosis of A375 and SK-MEL-28 cells. Effects of Linc00961 on the proliferation of (A) A375 and (B) SK-MEL-28 cells, as determined using a Cell Counting Kit-8 assay. (C) Effects of Linc00961 on the apoptosis of A375 and SK-MEL-28 cells, detected via flow cytometry. (D) Statistical analysis of the early apoptotic rates of A375 and SK-MEL-28 cells infected with Lv-control and Lv-Linc00961. (E) Tumor growth curve of A375 cells transfected with Lv-control and Lv-Linc00961. (F) Digital pictures of the resected tumors of animals injected with A375 cells infected with Lv-control (n=3) and Lv-Linc00961 (n=3), captured after 35 days. The maximum diameters of Lv-control and Lv-Linc00961 were 1.718 and 0.737 cm, respectively. Scale bar=1 cm. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. Lv-control. Linc00961, long intergenic noncoding RNA 00961; Lv, lentivirus; PI, propidium iodide.

Journal: International Journal of Oncology

Article Title: Linc00961 inhibits the proliferation and invasion of skin melanoma by targeting the miR-367/PTEN axis

doi: 10.3892/ijo.2019.4848

Figure Lengend Snippet: Effects of Linc00961 on the proliferation and apoptosis of A375 and SK-MEL-28 cells. Effects of Linc00961 on the proliferation of (A) A375 and (B) SK-MEL-28 cells, as determined using a Cell Counting Kit-8 assay. (C) Effects of Linc00961 on the apoptosis of A375 and SK-MEL-28 cells, detected via flow cytometry. (D) Statistical analysis of the early apoptotic rates of A375 and SK-MEL-28 cells infected with Lv-control and Lv-Linc00961. (E) Tumor growth curve of A375 cells transfected with Lv-control and Lv-Linc00961. (F) Digital pictures of the resected tumors of animals injected with A375 cells infected with Lv-control (n=3) and Lv-Linc00961 (n=3), captured after 35 days. The maximum diameters of Lv-control and Lv-Linc00961 were 1.718 and 0.737 cm, respectively. Scale bar=1 cm. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. Lv-control. Linc00961, long intergenic noncoding RNA 00961; Lv, lentivirus; PI, propidium iodide.

Article Snippet: Human A375 (cat. no. GCC-ME0001CS), SK-MEL-28 (cat. no GCC-ME0003CS) and A2058 (cat. no. GCC-ME0004CS) SM cell lines, and human epidermal HEMa-LP melanocytes (cat. no. GCC-NC0027CS) were all purchased from Shanghai GeneChem Co., Ltd., where they were characterized by mycoplasma detection, isozyme detection and DNA fingerprinting.

Techniques: Cell Counting, Flow Cytometry, Infection, Transfection, Injection

Effects of Linc00961 on the migration and invasion of SM cells. (A) Effects of Linc00961 on the migration of A375 and SK-MEL-28 cells, as determined by a Transwell assay. (B) Statistical analysis of the numbers of migratory A375 and SK-MEL-228 cells. (C) Effects of Linc00961 on cell invasion of A375 and SK-MEL-28 cells, as determined by a Matrigel-coated Transwell assay. (D) Statistical analysis of the numbers of invasive A375 and SK-MEL-228 cells. Scale bar=20 µ m. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. Lv-control. LINC00961, long intergenic noncoding RNA 00961; Lv, lentivirus.

Journal: International Journal of Oncology

Article Title: Linc00961 inhibits the proliferation and invasion of skin melanoma by targeting the miR-367/PTEN axis

doi: 10.3892/ijo.2019.4848

Figure Lengend Snippet: Effects of Linc00961 on the migration and invasion of SM cells. (A) Effects of Linc00961 on the migration of A375 and SK-MEL-28 cells, as determined by a Transwell assay. (B) Statistical analysis of the numbers of migratory A375 and SK-MEL-228 cells. (C) Effects of Linc00961 on cell invasion of A375 and SK-MEL-28 cells, as determined by a Matrigel-coated Transwell assay. (D) Statistical analysis of the numbers of invasive A375 and SK-MEL-228 cells. Scale bar=20 µ m. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. Lv-control. LINC00961, long intergenic noncoding RNA 00961; Lv, lentivirus.

Article Snippet: Human A375 (cat. no. GCC-ME0001CS), SK-MEL-28 (cat. no GCC-ME0003CS) and A2058 (cat. no. GCC-ME0004CS) SM cell lines, and human epidermal HEMa-LP melanocytes (cat. no. GCC-NC0027CS) were all purchased from Shanghai GeneChem Co., Ltd., where they were characterized by mycoplasma detection, isozyme detection and DNA fingerprinting.

Techniques: Migration, Transwell Assay

Linc00961 expression is inversely correlated with miR-367 expression in SM. Effects of Linc00961 on the expression of miRNAs that possessed putative binding sites for Linc00961 in (A) A375 and (B) SK-MEL-28 cells. (C) Expression levels of miR-367 in SM and BN tissues. (D) Association between miR-367 and Linc00961 in SM tissues. (E) Expression levels of miR-367 in SM cells and human epidermal melanocytes. (F) WT and MUT targeting regions of Linc00961 for miR-367. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. control (Lv-control, BN, HEMa-LP). Linc00961, long intergenic noncoding RNA 00961; miR, microRNA; SM, skin melanoma; BN, benign nevi; WT, wild-type; MUT, mutant.

Journal: International Journal of Oncology

Article Title: Linc00961 inhibits the proliferation and invasion of skin melanoma by targeting the miR-367/PTEN axis

doi: 10.3892/ijo.2019.4848

Figure Lengend Snippet: Linc00961 expression is inversely correlated with miR-367 expression in SM. Effects of Linc00961 on the expression of miRNAs that possessed putative binding sites for Linc00961 in (A) A375 and (B) SK-MEL-28 cells. (C) Expression levels of miR-367 in SM and BN tissues. (D) Association between miR-367 and Linc00961 in SM tissues. (E) Expression levels of miR-367 in SM cells and human epidermal melanocytes. (F) WT and MUT targeting regions of Linc00961 for miR-367. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. control (Lv-control, BN, HEMa-LP). Linc00961, long intergenic noncoding RNA 00961; miR, microRNA; SM, skin melanoma; BN, benign nevi; WT, wild-type; MUT, mutant.

Article Snippet: Human A375 (cat. no. GCC-ME0001CS), SK-MEL-28 (cat. no GCC-ME0003CS) and A2058 (cat. no. GCC-ME0004CS) SM cell lines, and human epidermal HEMa-LP melanocytes (cat. no. GCC-NC0027CS) were all purchased from Shanghai GeneChem Co., Ltd., where they were characterized by mycoplasma detection, isozyme detection and DNA fingerprinting.

Techniques: Expressing, Binding Assay, Mutagenesis

Linc00961 directly sponges miR-367 in SM cells. (A) Effects of Linc00961 on the expression of miR-367 in SM cells. Relative luciferase activity in (B) A375 and (C) SK-MEL-28 cells co-transfected with Linc00961-WT or LINC00961-MUT luciferase plasmid and miR-367 NC or mimics. (D) Detection of LINC00961 in RNA pulled down by Bio-Linc00961, Bio-Linc00961-MUT or Bio-NC probes. Detection of miR-367 in RNA pulled down by Bio-Linc00961, Bio-Linc00961-MUT or Bio-NC probes in (E) A375 and (F) SK-MEL-28 cells. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. control (Lv-control, miR-367 NC, Bio-NC-probe). Linc00961, long intergenic noncoding RNA 00961; miR, microRNA; SM, skin melanoma; NC, negative control; WT, wild-type; MUT, mutant; Bio, biotinylated.

Journal: International Journal of Oncology

Article Title: Linc00961 inhibits the proliferation and invasion of skin melanoma by targeting the miR-367/PTEN axis

doi: 10.3892/ijo.2019.4848

Figure Lengend Snippet: Linc00961 directly sponges miR-367 in SM cells. (A) Effects of Linc00961 on the expression of miR-367 in SM cells. Relative luciferase activity in (B) A375 and (C) SK-MEL-28 cells co-transfected with Linc00961-WT or LINC00961-MUT luciferase plasmid and miR-367 NC or mimics. (D) Detection of LINC00961 in RNA pulled down by Bio-Linc00961, Bio-Linc00961-MUT or Bio-NC probes. Detection of miR-367 in RNA pulled down by Bio-Linc00961, Bio-Linc00961-MUT or Bio-NC probes in (E) A375 and (F) SK-MEL-28 cells. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. control (Lv-control, miR-367 NC, Bio-NC-probe). Linc00961, long intergenic noncoding RNA 00961; miR, microRNA; SM, skin melanoma; NC, negative control; WT, wild-type; MUT, mutant; Bio, biotinylated.

Article Snippet: Human A375 (cat. no. GCC-ME0001CS), SK-MEL-28 (cat. no GCC-ME0003CS) and A2058 (cat. no. GCC-ME0004CS) SM cell lines, and human epidermal HEMa-LP melanocytes (cat. no. GCC-NC0027CS) were all purchased from Shanghai GeneChem Co., Ltd., where they were characterized by mycoplasma detection, isozyme detection and DNA fingerprinting.

Techniques: Expressing, Luciferase, Activity Assay, Transfection, Plasmid Preparation, Negative Control, Mutagenesis

Upregulation of miR-367 reverses the effects of Linc00961 on the proliferation and apoptosis of skin melanoma cells. (A) Expression of miR-367 in A375 and SK-MEL-28 cells transfected with Lv-control + miR-367 NC, Lv-Linc00961 + miR-367 NC or Lv-Linc00961 + miR-367 mimics, as determined via reverse transcription-quantitative PCR. Proliferation of (B) A375 and (C) SK-MEL-28 cells transfected with Lv-control + miR-367 NC, Lv-Linc00961 + miR-367 NC or Lv-Linc00961 + miR-367 mimics, as determined by a Cell Counting Kit-8 assay. (D) Apoptosis of A375 and SK-MEL-28 cells transfected with Lv-control + miR-367 NC, Lv-Linc00961 + miR-367 NC or Lv-Linc00961 + miR-367 mimics, as determined via flow cytometry. (E) Statistical analysis of early apoptotic rates of A375 and SK-MEL-228 cells transfected with Lv-control + miR-367 NC, Lv-Linc00961 + miR-367 NC or Lv-Linc00961 + miR-367 mimics. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. Lv-control + miR-367 NC. Linc00961, long intergenic noncoding RNA 00961; Lv, lentivirus; miR, microRNA; NC, negative control; PI, propidium iodide.

Journal: International Journal of Oncology

Article Title: Linc00961 inhibits the proliferation and invasion of skin melanoma by targeting the miR-367/PTEN axis

doi: 10.3892/ijo.2019.4848

Figure Lengend Snippet: Upregulation of miR-367 reverses the effects of Linc00961 on the proliferation and apoptosis of skin melanoma cells. (A) Expression of miR-367 in A375 and SK-MEL-28 cells transfected with Lv-control + miR-367 NC, Lv-Linc00961 + miR-367 NC or Lv-Linc00961 + miR-367 mimics, as determined via reverse transcription-quantitative PCR. Proliferation of (B) A375 and (C) SK-MEL-28 cells transfected with Lv-control + miR-367 NC, Lv-Linc00961 + miR-367 NC or Lv-Linc00961 + miR-367 mimics, as determined by a Cell Counting Kit-8 assay. (D) Apoptosis of A375 and SK-MEL-28 cells transfected with Lv-control + miR-367 NC, Lv-Linc00961 + miR-367 NC or Lv-Linc00961 + miR-367 mimics, as determined via flow cytometry. (E) Statistical analysis of early apoptotic rates of A375 and SK-MEL-228 cells transfected with Lv-control + miR-367 NC, Lv-Linc00961 + miR-367 NC or Lv-Linc00961 + miR-367 mimics. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. Lv-control + miR-367 NC. Linc00961, long intergenic noncoding RNA 00961; Lv, lentivirus; miR, microRNA; NC, negative control; PI, propidium iodide.

Article Snippet: Human A375 (cat. no. GCC-ME0001CS), SK-MEL-28 (cat. no GCC-ME0003CS) and A2058 (cat. no. GCC-ME0004CS) SM cell lines, and human epidermal HEMa-LP melanocytes (cat. no. GCC-NC0027CS) were all purchased from Shanghai GeneChem Co., Ltd., where they were characterized by mycoplasma detection, isozyme detection and DNA fingerprinting.

Techniques: Expressing, Transfection, Real-time Polymerase Chain Reaction, Cell Counting, Flow Cytometry, Negative Control

Upregulation of miR-367 reverses the effects of Linc00961 on the migration and invasion of SM cells. (A) Numbers of migratory A375 and SK-MEL-28 cells transfected with Lv-control + miR-367 NC, Lv-Linc00961 + miR-367 NC or Lv-Linc00961 + miR-367 mimics, determined by a Transwell assay. (B) Statistical analysis of the numbers of migratory A375 and SK-MEL-228 cells. (C) Numbers of invasive A375 and SK-MEL-28 cells transfected with Lv-control + miR-367 NC, Lv-Linc00961 + miR-367 NC or Lv-Linc00961 + miR-367 mimics, as determined by a Transwell assay. (D) Statistical analysis of the numbers of invasive A375 and SK-MEL-228 cells. Scale bar=20 µ m. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. Lv-control + miR-367 NC. Linc00961, long intergenic noncoding RNA 00961; Lv, lentivirus; miR, microRNA; NC, negative control.

Journal: International Journal of Oncology

Article Title: Linc00961 inhibits the proliferation and invasion of skin melanoma by targeting the miR-367/PTEN axis

doi: 10.3892/ijo.2019.4848

Figure Lengend Snippet: Upregulation of miR-367 reverses the effects of Linc00961 on the migration and invasion of SM cells. (A) Numbers of migratory A375 and SK-MEL-28 cells transfected with Lv-control + miR-367 NC, Lv-Linc00961 + miR-367 NC or Lv-Linc00961 + miR-367 mimics, determined by a Transwell assay. (B) Statistical analysis of the numbers of migratory A375 and SK-MEL-228 cells. (C) Numbers of invasive A375 and SK-MEL-28 cells transfected with Lv-control + miR-367 NC, Lv-Linc00961 + miR-367 NC or Lv-Linc00961 + miR-367 mimics, as determined by a Transwell assay. (D) Statistical analysis of the numbers of invasive A375 and SK-MEL-228 cells. Scale bar=20 µ m. Data from three experiments are presented as the mean ± SD. * P<0.05 vs. Lv-control + miR-367 NC. Linc00961, long intergenic noncoding RNA 00961; Lv, lentivirus; miR, microRNA; NC, negative control.

Article Snippet: Human A375 (cat. no. GCC-ME0001CS), SK-MEL-28 (cat. no GCC-ME0003CS) and A2058 (cat. no. GCC-ME0004CS) SM cell lines, and human epidermal HEMa-LP melanocytes (cat. no. GCC-NC0027CS) were all purchased from Shanghai GeneChem Co., Ltd., where they were characterized by mycoplasma detection, isozyme detection and DNA fingerprinting.

Techniques: Migration, Transfection, Transwell Assay, Negative Control

Silencing PTEN reverses the inhibitory effects of Linc00961 on the proliferation and invasion of SM cells. (A) mRNA and (B) protein expression of PTEN in A375 and SK MEL-28 cells transfected with Lv-control + miR-367 NC, Lv-Linc00961 + miR-367 NC or Lv-Linc00961 + miR-367 mimics. Proliferation of (C) A375 and (D) SK-MEL-28 cells transfected with Lv-control + siRNA NC, Lv-Linc00961 + siRNA NC or Lv-Linc00961 + PTEN siRNA, as determined by a Cell Counting Kit-8 assay. (E) Apoptosis of A375 and SK-MEL-28 cells transfected with Lv-control + siRNA NC, Lv-Linc00961 + siRNA NC or Lv-Linc00961 + PTEN siRNA, as determined via flow cytometry. Numbers of (F) migratory and (G) invasive A375 and SK-MEL-28 cells transfected with Lv-control + siRNA NC, Lv-Linc00961 + siRNA NC, or Lv-Linc00961 + PTEN siRNA, as determined via Transwell assays. Data from three experiments are presented as mean ± SD. * P<0.05 vs. Lv-control + miR-367 NC or siRNA NC. Linc00961, long intergenic noncoding RNA 00961; PTEN, phosphate and tension homology deleted on chromosome 10; Lv, lentivirus; miR, microRNA; siRNA, small interfering RNA; NC, negative control.

Journal: International Journal of Oncology

Article Title: Linc00961 inhibits the proliferation and invasion of skin melanoma by targeting the miR-367/PTEN axis

doi: 10.3892/ijo.2019.4848

Figure Lengend Snippet: Silencing PTEN reverses the inhibitory effects of Linc00961 on the proliferation and invasion of SM cells. (A) mRNA and (B) protein expression of PTEN in A375 and SK MEL-28 cells transfected with Lv-control + miR-367 NC, Lv-Linc00961 + miR-367 NC or Lv-Linc00961 + miR-367 mimics. Proliferation of (C) A375 and (D) SK-MEL-28 cells transfected with Lv-control + siRNA NC, Lv-Linc00961 + siRNA NC or Lv-Linc00961 + PTEN siRNA, as determined by a Cell Counting Kit-8 assay. (E) Apoptosis of A375 and SK-MEL-28 cells transfected with Lv-control + siRNA NC, Lv-Linc00961 + siRNA NC or Lv-Linc00961 + PTEN siRNA, as determined via flow cytometry. Numbers of (F) migratory and (G) invasive A375 and SK-MEL-28 cells transfected with Lv-control + siRNA NC, Lv-Linc00961 + siRNA NC, or Lv-Linc00961 + PTEN siRNA, as determined via Transwell assays. Data from three experiments are presented as mean ± SD. * P<0.05 vs. Lv-control + miR-367 NC or siRNA NC. Linc00961, long intergenic noncoding RNA 00961; PTEN, phosphate and tension homology deleted on chromosome 10; Lv, lentivirus; miR, microRNA; siRNA, small interfering RNA; NC, negative control.

Article Snippet: Human A375 (cat. no. GCC-ME0001CS), SK-MEL-28 (cat. no GCC-ME0003CS) and A2058 (cat. no. GCC-ME0004CS) SM cell lines, and human epidermal HEMa-LP melanocytes (cat. no. GCC-NC0027CS) were all purchased from Shanghai GeneChem Co., Ltd., where they were characterized by mycoplasma detection, isozyme detection and DNA fingerprinting.

Techniques: Expressing, Transfection, Cell Counting, Flow Cytometry, Small Interfering RNA, Negative Control